Tissue Culture Independent Transformation For Corc

Plant Cell Tiss Organ Cult DOI 10.1007/s11240-008-9447-0 authorized PAPER Tissue rageure independent conversion for Corchorus olitorius Abu Ashfaqur Sajib Æ Md. Shahidul Islam Æ Md. Shamim Reza Æ Arpita Bhowmik Æ Layla Fatema Æ Haseena khan legitimate: 27 May 2008 / Accepted: 15 howling(a) 2008 Ã" customs duty Science+Business Media B.V. 2008 Abstract In vitro regeneration is dif?cult for species of Corchorus and several transformation attempts ground on meander culture have failed. We describe a successful transformation protocol for C. olitorius victimization a technique independent of wander culture. Primary growth of a pose is brought round from the activities of meristematic share and subsequent division and differentiation of the differential cells into the tissues and organs of the plant. The leash behind the current study was that if about dividing cells in the meristematic portion become transformed by genus Agrobacterium and the cells re tain the dexterity for cell division, then the transgene(s) will be transferred to progeny cells, and if some of these cells later differentiate to form ? verbal buds, seeds generated from these buds will acquire the transgene(s) to next generation. In this study new jute plants were transformed at shoot apical meristematic region using Agrobacterium tumefaciens.

inheriting transmission of the transgene to progeny from genetically modi?ed plants was con?rmed by gus gene expression by histochemical analysis, extract on kanamycin containing medium, RTPCR, PCR ampli?cation and Southern hybridization. Ef?ciency of transformation was determined by selection ! on medium containing kanamycin, and inheritance of transgene to T2 generation plants. Keywords Jute Á mutation Á Agrobacterium tumefaciens Á In planta Introduction Abu Ashfaqur Sajib and Md. Shahidul Islam contributed equally to the study. A. A. Sajib Department of familial Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh Md. Shahidul Islam Á Md. Shamim...If you want to imbibe a full essay, dedicate it on our website: BestEssayCheap.com

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