To find out the optimum pH at which the enzyme catalase would best break down hydrogen peroxide.

GCSE SCIENCE COURSEWORK (BIOLOGY) PLANNING: Skill Area P make unnecessary down the level of your Investigation. The aim of investigation is that to find burst out the optimum pH at which the enzyme catalase would opera hat hold in down hydrogen peroxide. Briefly describe your advance investigate and include your results. Explain how these results involve helped you plan your of import examine (p8b). The purpose of the preliminary experiment was to find out the better(p) initiation of catalase for the investigation. The quartette choices of sources which were available were liver, apple, yeast and potato. to each 1 source was cut up into 1cm3 sections and placed in cardinal separate tryout tubes which each contained 5ml of H2O2. Observations were made on the physical evidence of what happened. Catalase source         Degree of bubbling         Number of times relights radiancy splint potato         normal      Â Â Â Â 1 apple         little         0 liver         a lot         7 yeast         Quite a lot         3 As the result show above, the potato was considered to be the best source to be physical exertiond in the real experiment be fountain it rised enough oxygen (but not too much(prenominal) to cause a dangerous situation). The apple did not break through to have sufficient catalase for the experiment as it did not produce each oxygen. The yeast and the liver, while rich in catalase unluckily prove to be over sufficient with an unnecessarily orotund make out of the enzyme needed. The oxygen is produced by this equation: rock all the apparatus that you at once plan to use (diagrams should be included) (P4b). 20ml of each buffer issue from pH3 to pH8 120ml of H2O2                                                       6 test tubes Three medium sized raw potatoe! s                           one stop up borer 50ml Manometer fluid                                    2x syringes 1x Ruler                                                      2x Pipettes 1x White tile                   ... If you indispensability to get a full essay, order it on our website: BestEssayCheap.com

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